Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/282318
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Title

A Temporary Immersion System to Improve Cannabis sativa Micropropagation

AuthorsRico, Saleta CSIC ORCID; Garrido, José; Sánchez, Conchi; Ferreiro-Vera, C.; Codesido Sampedro, Verónica CSIC; Vidal González, Nieves Pilar CSIC ORCID
KeywordsBioreactors
Liquid medium
Mass propagation
Plantform™
RITA
Sucrose
Issue Date23-Jun-2022
PublisherFrontiers Media
CitationFrontiers in Plant Science 13:895971 (2022)
AbstractThe aim of this study was to propagate axillary shoots of Cannabis sativa L. using liquid medium in temporary immersion bioreactors. The effect of immersion frequency (3 or 6 immersions per day), explant type (apical or basal sections), explant number (8, 10, and 16 explants), mineral medium (Murashige and Skoog half-strength nitrates, β-A and β-H, all supplemented with 2-μM metatopoline), sucrose supplementation (2, 0.5, and 0% sucrose), culture duration (4 and 6 weeks), and bioreactor type (RITA® and Plantform™) were investigated. As a result, we propose a protocol for the proliferation of cannabis apical segments in RITA® or Plantform™ bioreactors. The explants (8 per RITA® and 24 per Plantform™) are immersed for 1 min, 3 times per day in β-A medium supplemented with 2-μM metatopoline and 0.5% of sucrose and subcultured every 4 weeks. This is the first study using temporary immersion systems in C. sativa production, and our results provide new opportunities for the mass propagation of this species.
Publisher version (URL)http://dx.doi.org/10.3389/fpls.2022.895971
URIhttp://hdl.handle.net/10261/282318
DOI10.3389/fpls.2022.895971
Identifiersdoi: 10.3389/fpls.2022.895971
issn: 1664-462X
Appears in Collections:(MBG) Artículos




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