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Title

Ribosomal DNA, heterochromatin, and correlation with genome size in diploid and polyploid North American endemic sagebrushes (Artemisia, Asteraceae)

AuthorsGarcia, Sònia CSIC ORCID ; Garcia, Sònia CSIC ORCID ; Garnatje, Teresa CSIC ORCID CVN ; Pellicer, Jaume; Vallès, Joan; McArthur, E. D.; Siljak-Yakovlev, S.
KeywordsAllopolyploidy
Arabidopsis-type telomere
Autopolyploidy
Compositae
Chromomycin
DAPI
FISH
Fluorochrome banding
Genome organization
Tridentatae
Allopolyploıdie
Autopolyploıdie
Coloration differentielle aux fluorochromes
Organisation genomique
Issue Date2009
PublisherNational Research Council Canada
CitationGenome 52(12): 1012–1024 (2009)
Abstract[EN] Subgenus Tridentatae (Artemisia, Asteraceae) can be considered a polyploid complex. Both polyploidy and hybridization have been documented in the Tridentatae. Fluorescent in situ hybridization (FISH) and fluorochrome banding were used to detect and analyze ribosomal DNA changes linked to polyploidization in this group by studying four diploidpolyploid species pairs. In addition, genome sizes and heterochromatin patterns were compared between these populations. The linked 5S and 35S rRNA genes are confirmed as characteristic for Artemisia, and a pattern at the diploid level of three rDNA loci located at telomeric positions proved to be typical. Loss of rDNA loci was observed in some polyploids, whereas others showed additivity with respect to their diploid relatives. Genome downsizing was observed in all polyploids. Banding patterns differed depending on the pair of species analysed, but some polyploid populations showed an increased number of heterochromatic bands. FISH and fluorochrome banding were useful in determining the systematic position of Artemisia bigelovii, for which a differential pattern was found as compared with the rest of the group. Additionally, FISH was used to detect the presence of the Arabidopsis-type telomere repeat for the first time in Artemisia.
[FR]Le sous-genre Tridentatae (Artemisia, Asteraceae) peut eˆtre conside´re´ un complexe polyploı¨de. L’autopolyploı ¨die et l’allopolyploı¨die sont des facteurs bien documente´s comme ayant joue´ un roˆle dans l’e´volution de ces espe`ces. L’hybridation in situ fluorescente (FISH) et la coloration diffe´rentielle aux fluorochromes ont e´te´ utilise´es pour la de´tection et l’analyse des changements de l’ADN ribosomique qui sont rattache´s a` la polyploı¨disation chez ce groupe par le biais de l’e´tude de quatre couples diploı¨de-polyploı¨de. La taille du ge´nome et les mode`les de re´partition de l’he´te´rochromatine ont e´te´ aussi compare´s entre ces populations. Les ge`nes lie´s 5S et 35S sont confirme´s comme typiques d’Artemisia et un mode` le constitue´, au niveau diploı¨de, par trois loci de l’ADN ribosomique en position te´lome´rique s’est ave´re´ comme typique du sous-genre Tridentatae. Des pertes de loci de l’ADN ribosomique ont e´te´ observe´es chez quelques polyploı¨des, tandis que d’autres ont montre´ de l’additivite´ par rapport a` leurs diploı¨des. Une diminution de la taille du ge´nome haploı¨de a e´te´ observe´e chez tous les polyploı¨des. Les distributions des bandes marque´es aux fluorochromes sont diffe´rentes selon la paire d’espe`ces diploı¨de-polyploı¨de e´tudie´e, mais quelques populations polyploı¨des montrent un nombre plus e´leve´ de bandes he´te´rochromatiques. L’utilite´ taxonomique de l’analyse FISH et de la coloration diffe´rentielle aux fluorochromes est exploite´e pour e´lucider la position d’Artemisia bigelovii, ou` un mode`le particulier a e´te´ trouve´ en comparaison avec le reste du groupe. En plus, l’analyse FISH a servi a` de´tecter la pre´sence de la re´pe´tition te´lome´rique du type Arabidopsis pour la premie`re fois chez le genre.
Description13 p., il.
Publisher version (URL)http://dx.doi.org/10.1139/G09-077
URIhttp://hdl.handle.net/10261/28199
DOI10.1139/G09-077
ISSN0831-2796
Appears in Collections:(IBB) Artículos

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