Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/28010
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Influence of the linker type on the Au–S binding properties of thiol and disulfide-modified DNA self-assembly on polycrystalline gold

AuthorsMartínez-Orellana, Lidia CSIC ORCID ; Carrascosa, Laura G. CSIC ORCID; Huttel, Yves CSIC ORCID ; Lechuga, Laura M. CSIC ORCID ; Román García, Elisa Leonor CSIC
Issue Date7-Apr-2010
PublisherRoyal Society of Chemistry (UK)
CitationPhysical Chemistry Chemical Physics 12(13): 3301-3308 (2010)
AbstractWe investigate the Au–S binding properties of thiol and disulfide-modified DNA on polycrystalline gold by means of X-ray photoelectron spectroscopy in conditions close to dynamic processes of biosensors. The dependence of the immobilisation period on the quality and density of the self-assembly process of thiol (SH–(CH2)6–DNA), disulfide (DNA–CH2)6–SS–(CH2)6–DNA and DMTO–SS–(CH2)6–DNA) sulfur-modified oligonucleotide solutions (1 μM) that are employed for bioreceptor immobilisation is analysed. Two electronic components are found in the analysis of the S 2p core levels. One of them is clearly associated to thiolate formation, while the other can be associated to different origins. In order to identify the origin of this last component, a quantification of the non-specifically adsorbed species has been performed by rinsing the self-assembled monolayers (SAMs) with a mercapto hexanol (MCH) solution. It has been found that non-specifically adsorbed species contribute only partially to the appearance of this sulfur peak component in SAMs formed from disulfides. Electron bombardment was performed to study the evolution of this component as a consequence of surface degradation due to radiation effects. The results are also correlated with the possible presence of disulfides. We found that MCH is not stable during the measurements. The evolution of this compound and the possible causes for this behaviour are discussed.
Description8 páginas, 5 figuras.
Publisher version (URL)http://dx.doi.org/10.1039/B924504A
URIhttp://hdl.handle.net/10261/28010
DOI10.1039/B924504A
ISSN1463-9076
Appears in Collections:(CIN2) Artículos
(ICMM) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

WEB OF SCIENCETM
Citations

10
checked on May 22, 2022

Page view(s)

639
checked on May 22, 2022

Download(s)

117
checked on May 22, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.