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Título: | COX-2 Expression in Hepatocytes Improves Mitochondrial Function after Hepatic Ischemia-Reperfusion Injury |
Autor: | Fuertes-Agudo, Marina CSIC ORCID; Luque-Tévar, María CSIC ORCID; Cucarella, Carme CSIC ; Brea, Rocío CSIC; Boscá, Lisardo CSIC ORCID CVN ; Quintana-Cabrera, Ruben CSIC ORCID; Martín-Sanz, Paloma CSIC ORCID ; Casado, Marta CSIC ORCID | Palabras clave: | COX-2 High-resolution respirometry Ischemia-reperfusion Liver Mitochondrial dynamics Prostaglandins |
Fecha de publicación: | 30-ago-2022 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Antioxidants 11(9):1724 | Resumen: | Cyclooxygenase 2 (COX-2) is a key enzyme in prostanoid biosynthesis. The constitutive hepatocyte expression of COX-2 has a protective role in hepatic ischemia-reperfusion (I/R) injury (IRI), decreasing necrosis, reducing reactive oxygen species (ROS) levels, and increasing autophagy and antioxidant and anti-inflammatory response. The physiopathology of IRI directly impacts mitochondrial activity, causing ATP depletion and being the main source of ROS. Using genetically modified mice expressing human COX-2 (h-COX-2 Tg) specifically in hepatocytes, and performing I/R surgery on the liver, we demonstrate that COX-2 expression has a beneficial effect at the mitochondrial level. Mitochondria derived from h-COX-2 Tg mice livers have an increased respiratory rate associated with complex I electron-feeding pathways compared to Wild-type (Wt) littermates, without affecting complex I expression or assembly. Furthermore, Wt-derived mitochondria show a loss of mitochondrial membrane potential (ΔΨm) that correlates to increased proteolysis of fusion-related OPA1 through OMA1 protease activity. All these effects are not observed in h-COX-2 Tg mitochondria, which behave similarly to the Sham condition. These results suggest that COX-2 attenuates IRI at a mitochondrial level, preserving the proteolytic processing of OPA1, in addition to the maintenance of mitochondrial respiration. | Descripción: | 18 páginas, 7 figuras | Versión del editor: | https://dx.doi.org/10.3390/antiox11091724 | URI: | http://hdl.handle.net/10261/280010 | DOI: | 10.3390/antiox11091724 | E-ISSN: | 2076-3921 |
Aparece en las colecciones: | (IBV) Artículos (IC) Artículos (IIBM) Artículos |
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2022 Antioxidants 11-1724.pdf | 3,17 MB | Adobe PDF | Visualizar/Abrir |
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