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Surface plasmon resonance biosensors for highly sensitive detection in real samples

AuthorsSepúlveda, Borja ; Carrascosa, Laura G. ; Regatos, David ; Otte, Marinus A. ; Fariña, David ; Lechuga, Laura M.
KeywordsSurface plasmon resonance
DNA detection
Protein biosensing
Magneto-optic SPR
Localized surface plasmon resonance
Issue Date20-Aug-2009
PublisherThe International Society for Optics and Photonics
CitationProceedings of SPIE 7397: 7397OY (2009)
AbstractIn this work we summarize the main results obtained with the portable surface plasmon resonance (SPR) device developed in our group (commercialised by SENSIA, SL, Spain), highlighting its applicability for the real-time detection of extremely low concentrations of toxic pesticides in environmental water samples. In addition, we show applications in clinical diagnosis as, on the one hand, the real-time and label-free detection of DNA hybridization and single point mutations at the gene BRCA-1, related to the predisposition in women to develop an inherited breast cancer and, on the other hand, the analysis of protein biomarkers in biological samples (urine, serum) for early detection of diseases. Despite the large number of applications already proven, the SPR technology has two main drawbacks: (i) not enough sensitivity for some specific applications (where pM-fM or single-molecule detection are needed) (ii) low multiplexing capabilities. In order solve such drawbacks, we work in several alternative configurations as the Magneto-optical Surface Plasmon Resonance sensor (MOSPR) based on a combination of magnetooptical and ferromagnetic materials, to improve the SPR sensitivity, or the Localized Surface Plasmon Resonance (LSPR) based on nanostructures (nanoparticles, nanoholes,...), for higher multiplexing capabilities.
Description11 páginas, 9 figuras.-- Trabajo presentado en la conferencia "Biosensing II", 4-Agosto-2009, San Diego, CA, USA; Editores: Manijeh Razeghi, Hooman Mohseni.
Publisher version (URL)http://dx.doi.org/10.1117/12.827062
Appears in Collections:(CIN2) Artículos
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