English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/27962
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Modulation of Proteins Adsorption onto the Surface of Chitosan Complexed with Anionic Copolymers. Real Time Analysis by Surface Plasmon Resonance

AuthorsAguilar, María Rosa ; Gallardo Ruiz, Alberto ; Lechuga, Laura M. ; Calle Martín, Ana ; San Román, Julio
KeywordsAlbumin
Chitosan
Fibrinogen
Polyelectrolytes
Surface plasmon resonance
Issue Date28-Jun-2004
PublisherWiley-Blackwell
CitationMacromolecular Bioscience 4(7): 631-638 (2004)
AbstractThe interpolyelectrolyte complex formation between chitosan and anionic polyacrylic derivatives, bearing sulfonic moieties, as well as the protein adsorption onto the chitosan/polyacrylic complexes were studied by surface plasmon resonance (SPR) optical biosensor. This unique technique allows a real time monitoring of different surface molecular interactions with very high sensitivity. The acrylic macromolecules are two families of copolymers of 2-acrylamido-2-methylpropane sulfonic acid (AMPS) and, respectively, 2-hydroxyethylmethacrylate (HEMA) and N,N′-dimethylacrylamide (DMAA). The complexation process was evaluated through the SPR measurements resulting from the flowing of polyacrylic aqueous solution over the sensor previously coated with chitosan. The SPR was able to differentiate strong ionic bonds from other weak and reversible interactions. By means of the coated sensors (uncomplexed and the whole series of complexed chitosan), SPR cold be used for a simple “in vitro” protein adsorption analysis, by flowing aqueous solutions of albumin and fibrinogen. While both proteins were adsorbed on the uncomplexed chitosan, the complexed coatings exhibited different and very promising behaviors. In particular, they showed no adsorption or only selective adsorption of albumin.
Description8 páginas, 11 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1002/mabi.200400039
URIhttp://hdl.handle.net/10261/27962
DOI10.1002/mabi.200400039
ISSN1616-5187
Appears in Collections:(ICTP) Artículos
(IMN-CNM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.