English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/2792
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 52 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Grabbing the message: structural basis of mRNA 3′UTR recognition by Hrp1

AutorPérez Cañadillas, José Manuel
Palabras claveCleavage and Polyadenylation
Hrp1
Nuclear Magnetic Resonance
RNA-binding Proteins
RNA Processing
Fecha de publicación22-jun-2006
EditorJohn Wiley & Sons
CitaciónEMBO Journal 25(13): 3167–3178 (2006)
ResumenThe recognition of specific signals encoded within the 3′-untranslated region of the newly transcribed mRNA triggers the assembly of a multiprotein machine that modifies its 3′-end. Hrp1 recognises one of such signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The molecular bases of this interaction are revealed here by the solution structure of a complex between Hrp1 and an oligonucleotide mimicking the PEE. Six consecutive bases (AUAUAU) are specifically recognised by two RNA-binding domains arranged in tandem. Both protein and RNA undergo significant conformational changes upon complex formation with a concomitant large surface burial of RNA bases. Key aspects of RNA specificity can be explained by the presence of intermolecular aromatic–aromatic contacts and hydrogen bonds. Altogether, the Hrp1–PEE structure represents one of the first steps towards understanding of the assembly of the cleavage and polyadenylation machinery at the atomic level.
DescripciónAuthor affiliation (other than CSIC): Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
URIhttp://hdl.handle.net/10261/2792
DOI10.1038/sj.emboj.7601190
ISSN0261-4189
Aparece en las colecciones: (IQFR) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.