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Dopaminergic regulation of the serotonergic raphe-striatal pathway: microdialysis studies in freely moving rats

AuthorsFerré, Sergi; Cortés, Roser CSIC ORCID; Artigas, Francesc CSIC ORCID
Issue DateAug-1994
PublisherSociety for Neuroscience
CitationJournal of Neuroscience 14(8): 4839-4846 (1994)
AbstractMorphological evidence demonstrates the existence of dopaminergic afferent pathways and dopamine (DA)-containing neurons in the dorsal raphe nucleus (DRN). In a recent report, a DA D,-like receptor-mediated regulation of serotonin (5-HT) extracellular concentration in DRN has been found. Given the existence of somatodendritic 5-HT,, autoreceptors in the DRN, changes of the extracellular concentration of 5-HT in the vicinity of cell bodies and dendrites may be relevant for the control of the activity of ascending serotonergic pathways. In the present brain microdialysis study we have used a chromatographic method (HPLC) enabling the simultaneous measurement of DA, 5-HT, and their main metabolites dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA). The presence of a neuronal pool of DA within the DRN was revealed by the local infusion of amphetamine (10 PM), which significantly increased the extracellular concentration of both amines. The local striatal infusion (10 PM) of the selective DA D,-like agonist SKF-38393, the selective DA D,-like agonist quinpirole (LY 171,555), or the nonselective DA agonist apomorphine markedly decreased DA and DOPAC extracellular concentrations and failed to modify 5-HT or 5-HIAA in the striatum, indicating the lack of terminal (striatal) control of 5-HT release by dopaminergic transmission. In contrast, the systemic administration of apomorphine (2.8 wmol/kg, s.c.) significantly increased the extracellular concentration of 5-HT in the DRN and decreased it in the striatum. The reduction of striatal 5-HT extracellular concentration was prevented by the previous administration of the selective 5-HT, receptor antagonist WAY 100135 (17.6 rmol/kg, s.c.), which by itself did not change extracellular 5-HT in striatum. These results support that dopaminergic neurotransmission inhibits the activity of DRN-striatal neurons by increasing 5-HT extracellular concentration in DRN and, consequently, by increasing somatodendritic 5-HT,, autoreceptor stimulation in this nucleus.
Description8 pages, 8 figures.-- PMID: 7519257 [PubMed].
Publisher version (URL)http://www.jneurosci.org/cgi/reprint/14/8/4839
Appears in Collections:(IQAC) Artículos
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