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Title

H2S in horticultural plants: endogenous detection by an electrochemical sensor, emission by a gas detector, and Its correlation with L-cysteine desulfhydrase (LCD) activity

AuthorsMuñoz-Vargas, María A. CSIC ORCID; González-Gordo, Salvador CSIC ORCID; Palma Martínez, José Manuel CSIC ORCID; Corpas, Francisco J. CSIC ORCID
KeywordsAllium
Hydrogen sulfide
Garlic
Gas detector
Ion-selective microelectrode
L-cysteine desulfhydrase
Isozymes
Issue Date2022
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 23(10): 5648 (2022)
AbstractH S has acquired great attention in plant research because it has signaling functions under physiological and stress conditions. However, the direct detection of endogenous H S and its potential emission is still a challenge in higher plants. In order to achieve a comparative analysis of the content of H S among different plants with agronomical and nutritional interest including pepper fruits, broccoli, ginger, and different members of the genus Allium such as garlic, leek, Welsh and purple onion, the endogenous H S and its emission was determined using an ion-selective microelectrode and a specific gas detector, respectively. The data show that endogenous H S content range from pmol to µmol H S · g fresh weight whereas the H S emission of fresh-cut vegetables was only detected in the different species of the genus Allium with a maximum of 9 ppm in garlic cloves. Additionally, the activity and isozymes of the L-cysteine desulfhydrase (LCD) were analyzed, which is one of the main enzymatic sources of H S, where the different species of the genus Allium showed the highest activities. Using non-denaturing gel electrophoresis, the data indicated the presence of up to nine different LCD isozymes from one in ginger to four in onion, leek, and broccoli. In summary, the data indicate a correlation between higher LCD activity with the endogenous H S content and its emission in the analyzed horticultural species. Furthermore, the high content of endogenous H S in the Allium species supports the recognized benefits for human health, which are associated with its consumption.
Publisher version (URL)http://dx.doi.org/10.3390/ijms23105648
URIhttp://hdl.handle.net/10261/275910
DOI10.3390/ijms23105648
Identifiersdoi: 10.3390/ijms23105648
issn: 1422-0067
Appears in Collections:(EEZ) Artículos




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