Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/27547
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dc.contributor.authorPueyo, José Javier-
dc.contributor.authorAlfonso Lozano, Miguel-
dc.contributor.authorAndrés, Carmen-
dc.contributor.authorPicorel Castaño, Rafael-
dc.date.accessioned2010-09-10T07:40:28Z-
dc.date.available2010-09-10T07:40:28Z-
dc.date.issued2002-
dc.identifier.citationBiochimica et Biophysica Acta 1554: 29-35 (2002)en_US
dc.identifier.issn0005-2728-
dc.identifier.urihttp://hdl.handle.net/10261/27547-
dc.description7 pages, figures, and tables statistics.en_US
dc.description.abstractPhotosynthetic oxygen evolution is an extremely heat-sensitive process and incubation of spinach Photosystem II (PSII) membranes at 40ºC for only several minutes leads to its complete inactivation. Substitution experiments of the spinach 33-kDa manganese stabili<ing protein by a homologue protein, isolated either from the thermophilic cyanobacterium Phormidium laminosum, or from Escherichia coli as a recombinant thermophilic cyanobacterial protein, showed a significant increase in tolerance to heat inactivation of the oxigen-evolving activity. The results allow us to suggest that thermalinactivation of oxygen evolution in higher plant PSII membranes is due to dissociation of the 33-kDa protein as a consequence of temperature-induced conformational changes, and stabilization can be provided by sustitution by a thermostable homologue whose secondary structure and binding to PSII remain ulatered at moderately hight temperature.en_US
dc.format.extent22195 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsclosedAccessen_US
dc.subjectPhotosystem IIen_US
dc.subjectHeat inactivationen_US
dc.subject33-kDa proteinen_US
dc.subjectManganese stabilizing proteinen_US
dc.subjectOxygen evolutionen_US
dc.titleIncreased tolerance to thermal inactivation of oxygen evolution in spinach Photosystem II membranes by sustitution of the extrinsic 33-KDa protein by its homologue from a thermophilic cyanobacteriumen_US
dc.typeartículoen_US
dc.description.peerreviewedPeer revieweden_US
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.openairetypeartículo-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
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