Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/269523
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides

AutorCaño, Rafael del; García-Mendiola, Tania; García-Nieto, Daniel CSIC ORCID; Álvaro Bruna, Raquel CSIC ORCID ; Luna, Mónica CSIC ORCID; Alarcón Iniesta, Hernán; Coloma, Rocío; Rodríguez Diaz, Ciro; Milán-Rois, Paula; Castellanos, Milagros; Abreu, Melanie; Cantón, Rafael; Galán, Juan Carlos; Pineda, Teresa; Pariente, Félix; Miranda, Rodolfo CSIC ORCID; Somoza, Álvaro; Lorenzo, Encarnación
Palabras claveAuNTs
Biosensors
SARS-CoV-2
Amplification-free detection
Fecha de publicación1-abr-2022
EditorSpringer Nature
CitaciónMicrochimica Acta 189: 171 (2022)
ResumenGold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment.
Versión del editorhttps://doi.org/10.1007/s00604-022-05272-y
URIhttp://hdl.handle.net/10261/269523
DOI10.1007/s00604-022-05272-y
ISSN0026-3672
E-ISSN1436-5073
Aparece en las colecciones: (PTI Salud Global) Colección Especial COVID-19
(IMN-CNM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Caño2022_Article_Amplification-freeDetectionOfS.pdf1,96 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

16
checked on 25-mar-2024

WEB OF SCIENCETM
Citations

15
checked on 28-feb-2024

Page view(s)

63
checked on 29-mar-2024

Download(s)

52
checked on 29-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Este item está licenciado bajo una Licencia Creative Commons Creative Commons