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Título: | Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides |
Autor: | Caño, Rafael del; García-Mendiola, Tania; García-Nieto, Daniel CSIC ORCID; Álvaro Bruna, Raquel CSIC ORCID ; Luna, Mónica CSIC ORCID; Alarcón Iniesta, Hernán; Coloma, Rocío; Rodríguez Diaz, Ciro; Milán-Rois, Paula; Castellanos, Milagros; Abreu, Melanie; Cantón, Rafael; Galán, Juan Carlos; Pineda, Teresa; Pariente, Félix; Miranda, Rodolfo CSIC ORCID; Somoza, Álvaro; Lorenzo, Encarnación | Palabras clave: | AuNTs Biosensors SARS-CoV-2 Amplification-free detection |
Fecha de publicación: | 1-abr-2022 | Editor: | Springer Nature | Citación: | Microchimica Acta 189: 171 (2022) | Resumen: | Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment. | Versión del editor: | https://doi.org/10.1007/s00604-022-05272-y | URI: | http://hdl.handle.net/10261/269523 | DOI: | 10.1007/s00604-022-05272-y | ISSN: | 0026-3672 | E-ISSN: | 1436-5073 |
Aparece en las colecciones: | (PTI Salud Global) Colección Especial COVID-19 (IMN-CNM) Artículos |
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Caño2022_Article_Amplification-freeDetectionOfS.pdf | 1,96 MB | Adobe PDF | Visualizar/Abrir |
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