Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/268449
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds |
Autor: | Albuquerque, André; Óvilo Martín, Cristina; Núñez, Yolanda; Benítez Yáñez, Rita María; López-García, Adrián; Suárez García, Fabián CSIC ORCID ; Félix, Maria do Rosário; Laranjo, Marta; Charneca, Rui; Martins, José Manuel | Palabras clave: | Alentejano pig Bísaro pig MAP3K14 MYH3 MYH7 Intramuscular fat Meat quality Skeletal muscle Transcriptomes |
Fecha de publicación: | 16-may-2021 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Animals 11(6):1423 (2021) | Resumen: | Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14's coding product NIK, failing to restore insulin sensitivity. | Descripción: | 21 Págs. This article belongs to the Special Issue Transcriptomics in Autochthonous Breeds or Populations of Livestock and Aquaculture Species. | Versión del editor: | https://doi.org/10.3390/ani11051423 | URI: | http://hdl.handle.net/10261/268449 | DOI: | 10.3390/ani11051423 | ISSN: | 2076-2615 |
Aparece en las colecciones: | (INIA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Transcriptomic-Profiling-of-Skeletal.pdf | artículo | 6,65 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
7
checked on 20-abr-2024
SCOPUSTM
Citations
15
checked on 19-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 28-feb-2024
Page view(s)
50
checked on 23-abr-2024
Download(s)
52
checked on 23-abr-2024