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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Redondo, F. J. | - |
dc.contributor.author | Coba de la Peña, Teodoro | - |
dc.contributor.author | Morcillo, César N. | - |
dc.contributor.author | Lucas, M. Mercedes | - |
dc.contributor.author | Pueyo, José Javier | - |
dc.date.accessioned | 2010-08-06T07:31:23Z | - |
dc.date.available | 2010-08-06T07:31:23Z | - |
dc.date.issued | 2009-02 | - |
dc.identifier.citation | Plant Physiology 149(1166-1178 (2009) | en_US |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | http://hdl.handle.net/10261/26827 | - |
dc.description | 12 pages, photos, figures, and tables statistics. | en_US |
dc.description.abstract | Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corresponding control bacteria. The decline of nitrogenase activity and the nodule structural and ultrastructural alterations that are associated with nodule senescence were significantly delayed in flavodoxinexpressing nodules. Substantial changes in nodule antioxidant metabolism, involving antioxidant enzymes and ascorbateglutathione cycle enzymes and metabolites, were detected in flavodoxin-containing nodules. Lipid peroxidation was also significantly lower in flavodoxin-expressing nodules than in control nodules. The observed amelioration of the oxidative balance suggests that the delay in nodule senescence was most likely due to a role of the protein in reactive oxygen species detoxification. Flavodoxin overexpression also led to high starch accumulation in nodules, without reduction of the nitrogenfixing activity. | en_US |
dc.description.sponsorship | We thank Dr. M.F. Fillat for the kind gift of plasmid pTrc99a-Fld and F. Pinto and E. Gonza´lez for technical assistance. | en_US |
dc.format.extent | 1522842 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | American Society of Plant Biologists | en_US |
dc.rights | openAccess | en_US |
dc.subject | Alfalfa | en_US |
dc.subject | Flavodoxin | en_US |
dc.subject | Bacteroids Induces | en_US |
dc.subject | Root Nodules | en_US |
dc.title | Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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2009 Redondo Plant Physiology.pdf | 1,49 MB | Adobe PDF | Visualizar/Abrir |
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