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Título

Thrombin-Binding Aptamer Quadruplex Formation: AFM and Voltammetric Characterization

AutorDiculescu, Victor Constantin; Chiorcea-Paquim, Ana-Maria; Eritja Casadellà, Ramón; Oliveira-Bret, Ana Maria
Fecha de publicación2010
EditorSage Publications
CitaciónJournal of Nucleic Acids 2010 (2010)
ResumenThe adsorption and the redox behaviour of thrombin-binding aptamer (TBA) and extended TBA (eTBA) were studied using atomic force microscopy and voltammetry at highly oriented pyrolytic graphite and glassy carbon. The different adsorption patterns and degree of surface coverage were correlated with the sequence base composition, presence/absence of K+, and voltammetric behaviour of TBA and eTBA. In the presence of K+, only a few single-stranded sequences present adsorption, while the majority of the molecules forms stable and rigid quadruplexes with no adsorption. Both TBA and eTBA are oxidized and the only anodic peak corresponds to guanine oxidation. Upon addition of K+ ions, TBA and eTBA fold into a quadruplex, causing the decrease of guanine oxidation peak and occurrence of a new peak at a higher potential due to the oxidation of G-quartets. The higher oxidation potential of G-quartets is due to the greater difficulty of electron transfer from the inside of the quadruplex to the electrode surface than electron transfer from the more flexible single strands.
Descripción8 pages, 4 figures, 2 schemes.
Versión del editorhttp://dx.doi.org/10.4061/2010/841932
URIhttp://hdl.handle.net/10261/26818
DOI10.4061/2010/841932
ISSN2090-021X (Online)
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