Please use this identifier to cite or link to this item:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Significance of PD1 Alternative Splicing in Celiac Disease as a Novel Source for Diagnostic and Therapeutic Target

AuthorsPonce de León, Candelaria; Lorite, Pedro; López-Casado, Miguel Ángel; Barro Losada, Francisco CSIC ORCID ; Palomeque, Teresa; Torres, María Isabel
Celiac disease
Alternative splicing
Gluten peptides
Immune checkpoint
Issue Date16-Jun-2021
PublisherFrontiers Media
CitationFrontiers in Immunology 12: 678400 (2021)
Abstract[Background] We have focused on the alteration of the PD-1/PD-L1 pathway in celiac disease and discussed the roles of the PD1 pathway in regulating the immune response. We explored the idea that the altered mRNA splicing process in key regulatory proteins could represent a novel source to identify diagnostic, prognostic, and therapeutic targets in celiac disease.
[Methods] We characterized the PD1 mRNA variants’ profile in CD patients and in response to gluten peptides’ incubation after in vitro experiments. Total RNA from whole blood was isolated, and the coding region of the human PD-1 mRNA was amplified by cDNA PCR.
[Results] PCR amplification of the human PD-1 coding sequence revealed an association between the over-expression of the sPD-1 protein and the PD-1Δex3 transcript in celiac disease. Thus, we have found three novel alternative spliced isoforms, two of which result in a truncated protein and the other isoform with a loss of 14 aa of exon 2 and complete exon 3 (Δ3) which could encode a new soluble form of PD1 (sPD-1).
[Conclusions] Our study provides evidence that dietary gluten can modulate processes required for cell homeostasis through the splicing of pre-mRNAs encoding key regulatory proteins, which represents an adaptive mechanism in response to different nutritional conditions.
Publisher version (URL)
Identifiersdoi: 10.3389/fimmu.2021.678400
e-issn: 1664-3224
Appears in Collections:(IAS) Artículos

Files in This Item:
File Description SizeFormat
PD1_Alternative.pdf12,14 MBAdobe PDFThumbnail
Show full item record
Review this work

Page view(s)

checked on Aug 18, 2022


checked on Aug 18, 2022

Google ScholarTM


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.