Comparison of the influence of different extraction procedures and analytical methods on the qualitative and quantitative results for PSP toxins determination

Abstract

Using toxic mussels from outbreaks of G. catenatum, the results of PSP intercalibrations and PSP toxin evaluation are presented between laboratories responsible for monitoring shellfish production areas in Cataluña, Galicia and Andalucía. Different extraction and clean up procedures are evaluated. PSP toxicity was estimated from HPLC analyses with pre-column and post-column oxidation and compared with the reference method. HCl 0.1 N can be considered a universal extractant since it can be used in both Mouse Bioassay (MBA) and HPLC with pre-column or post-column oxidation. On the other hand, using pre-column methods it is not possible resolve pairs of toxins with different toxicity and quantitative results are underestimated relative to MBA