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Título: | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
Autor: | Schvartzman, Jorge Bernardo CSIC ORCID ; Martínez, Víctor; Hernandez, Pablo CSIC ; Krimer, Dora B. CSIC ORCID ; Fernández-Nestosa, María José | Palabras clave: | Catenation DNA chirality DNA topology Pre-catenation Replication Supercoiling |
Fecha de publicación: | 25-feb-2021 | Editor: | John Wiley & Sons | Citación: | BioEssays | Resumen: | The topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates,taking into account the progression of replication forks. The high processivity of Topo IV to relax the left-handed (+) supercoiling that transiently accumulates ahead of the forks is not essential, since DNA gyrase and swiveling of the forks cooperate with Topo IV to accomplish this task in vivo. We conclude that despite Topo IV has a lower processivity to unlink the right-handed (+) crossings of pre-catenanes and fully replicated catenanes, this is indeed itsmain role in vivo. This would explain why in the absence of Topo IV replication goes-on, but fully replicated sister duplexes remain heavily catenated. | Descripción: | 7 p.-4 fig. | Versión del editor: | https://doi.org/10.1002/bies.202000309 | URI: | http://hdl.handle.net/10261/263966 | DOI: | 10.1002/bies.202000309 | ISSN: | 0265-9247 | E-ISSN: | 1521-1878 |
Aparece en las colecciones: | (CIB) Artículos |
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Bioessays_Schvartzman_2021.pdf | Artículo principal | 2,96 MB | Adobe PDF | Visualizar/Abrir |
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