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Título: | Genetic insights into the dark matter of the mammalian gut microbiota through targeted genome reconstruction |
Autor: | Lugli, Gabriele Andrea; Alessandri, Giulia; Milani, Christian; Viappiani, Alice; Fontana, Federico; Tarracchini, Chiara; Mancabelli, Leonardo; Argentini, Chiara; Ruíz García, Lorena CSIC ORCID ; Margolles Barros, Abelardo CSIC ORCID; Sinderen, Douwe van; Turroni, Francesca; Ventura, Marco | Fecha de publicación: | jun-2021 | Editor: | Society for Applied Microbiology John Wiley & Sons |
Citación: | Environmental Microbiology 23(6): 3294-3305 (2021) | Resumen: | Whole metagenomic shotgun (WMS) sequencing has dramatically enhanced our ability to study microbial genomics. The possibility to unveil the genetic makeup of bacteria that cannot be easily isolated has significantly expanded our microbiological horizon. Here, we report an approach aimed at uncovering novel bacterial species by the use of targeted WMS sequencing. Employing in silico data retrieved from metabolic modelling to formulate a chemically defined medium (CDM), we were able to isolate and subsequently sequence the genomes of six putative novel species of bacteria from the gut of non-human primates. | Versión del editor: | http://dx.doi.org/10.1111/1462-2920.15559 | URI: | http://hdl.handle.net/10261/263545 | DOI: | 10.1111/1462-2920.15559 | Identificadores: | doi: 10.1111/1462-2920.15559 e-issn: 1462-2920 issn: 1462-2912 |
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Environmental Microbiology - 2021 - Lugli - Genetic insights into the dark matter of the mammalian gut microbiota through.pdf | 1,46 MB | Adobe PDF | Visualizar/Abrir |
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