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Título: | C. elegans as a Nestor Guillermo progeria syndrome model |
Autor: | Romero Bueno, Raquel CSIC ORCID; Rojas, Marta CSIC; Ayuso, Cristina CSIC ORCID; Dobrzynska, Agnieszka CSIC; Riedel, Christian; Askjaer, Peter CSIC ORCID | Fecha de publicación: | 17-may-2021 | Citación: | EMBL Conference: Chromatin and Epigenetics (2021) | Resumen: | BAF-1 (Barrier to Autointegration Factor) is a highly conserved chromatin binding protein implicated in nuclear envelope (NE) breakdown, assembly and repair as well as chromatin compaction. It acts as a homodimer and its NE localisation is interdependent of lamins and LEM-domain proteins (LAP2, emerin, and MAN1). Nevertheless, BAF-1 is also present in the nucleoplasm. Strikingly, a single amino acid substitution in human BAF (A12T) causes Nestor-Guillermo Progeria Syndrome (NGPS). This premature ageing illness affects a variety of tissues, leading to growth retardation, severe skeletal defects and scoliosis. We have modified the baf-1 locus in Caenorhabditis elegans to mimic the human NGPS mutation (baf-1(G12T)) to elucidate why a mutation in an essential protein expressed throughout development triggers the appearance of symptoms ~2 years after birth. We report that NE levels of lamin/LMN-1 and emerin/EMR-1 are reduced in baf-1(G12T) mutants, whereas errors in chromosome segregation are increased. Although the baf-1(G12T) mutation does not affect lifespan, age-dependent nuclear morphology deterioration is accelerated in mutant animals. Moreover, we found that baf-1(G12T) mutants are hypersensitive to NE perturbations, particularly to modifications affecting lamin/LMN-1. Using Bimolecular Fluorescence Complementation (BiFC), we discovered that the interaction of the BAF-1(G12T) protein with itself is weaker when compared to wild type BAF-1 homo-dimerisation. Our BiFC experiments also revealed novel chromatin interaction partners. To explore if the NGPS mutation affects BAF-1¿s association with chromatin, we determined the binding profiles for wild type and mutant BAF-1 through tissue-specific DamID. Globally, the profiles for the two proteins are very similar, but we also identified discrete genomic regions with altered association to BAF-1. We are currently correlating these observations with tissue-specific changes in gene expression. | Descripción: | Trabajo presentado en EMBL Conference: Chromatin and Epigenetics, celebrada en modalidad virtual del 17 al 20 de mayo de 2021. | URI: | http://hdl.handle.net/10261/263408 |
Aparece en las colecciones: | (CABD) Comunicaciones congresos |
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