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Título: | Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure |
Autor: | Vázquez Prol, Francisco; Marquez-Molins, Joan CSIC ORCID CVN; Rodrigo, Ismael CSIC ORCID ; López-Gresa, Pilar CSIC ORCID ; Bellés Albert, José-María CSIC ORCID; Gómez, Gustavo CSIC ORCID ; Pallás Benet, Vicente CSIC ORCID; Lisón Párraga, Purificación CSIC ORCID | Palabras clave: | Pospiviroidae Stress CEVd Ribosome TCDVd Tomato PSTVd Eggplant Agro-infiltration Transcript |
Fecha de publicación: | 2021 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 22(12): 6189 (2021) | Resumen: | Infectious viroid clones consist of dimeric cDNAs used to generate transcripts which mimic the longer-than-unit replication intermediates. These transcripts can be either generated in vitro or produced in vivo by agro-inoculation. We have designed a new plasmid, which allows both inoculation methods, and we have compared them by infecting Solanum lycopersicum and Solanum melongena with clones of Citrus exocortis virod (CEVd), Tomato chlorotic dwarf viroid (TCDVd), and Potato spindle tuber viroid (PSTVd). Our results showed more uniform and severe symptoms in agro-inoculated plants. Viroid accumulation and the proportion of circular and linear forms were different depending on the host and the inoculation method and did not correlate with the symptoms, which correlated with an increase in PR1 induction, accumulation of the defensive signal molecules salicylic (SA) and gentisic (GA) acids, and ribosomal stress in tomato plants. The alteration in ribosome biogenesis was evidenced by both the upregulation of the tomato ribosomal stress marker SlNAC082 and the impairment in 18S rRNA processing, pointing out ribosomal stress as a novel signature of the pathogenesis of nuclear-replicating viroids. In conclusion, this updated binary vector has turned out to be an efficient and reproducible method that will facilitate the studies of viroid–host interactions. | Descripción: | This article belongs to the Section Molecular Plant Sciences. | Versión del editor: | https://doi.org/10.3390/ijms22126189 | URI: | http://hdl.handle.net/10261/262317 | DOI: | 10.3390/ijms22126189 | E-ISSN: | 1422-0067 |
Aparece en las colecciones: | (I2SysBio) Artículos (IBMCP) Artículos |
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