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dc.contributor.authorLatorre-Pérez, Adrieles_ES
dc.contributor.authorGimeno-Valero, Helenaes_ES
dc.contributor.authorTanner, Kristiees_ES
dc.contributor.authorPascual, Javieres_ES
dc.contributor.authorVilanova, Cristinaes_ES
dc.contributor.authorPorcar, Manueles_ES
dc.date.accessioned2022-02-25T08:12:23Z-
dc.date.available2022-02-25T08:12:23Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Microbiology 12: 768240 (2021)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/261984-
dc.description.abstractBioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile laboratory. Results enabled the identification of locations showing the greatest and the least potential, and a second, informed sampling was performed focusing on those sites. After finishing the expedition, a culture collection of 166 strains belonging to 50 different genera was established. Overall, Nanopore and culturing data correlated well, since samples holding a greater potential at the microbiome level also yielded a more interesting set of microbial isolates, whereas samples showing less biodiversity resulted in a reduced (and redundant) set of culturable bacteria. Thus, we anticipate that portable sequencers hold potential as key, easy-to-use tools for in situ-informed bioprospecting strategies.es_ES
dc.description.sponsorshipFinancial support from the Spanish Government grant SETH (ref: RTI2018-095584-B-C41-42-43-44) is acknowledged. AL-P is a recipient of a Doctorado Industrial fellowship from the Spanish Ministerio de Ciencia, Innovación y Universidades (reference DI-17-09613).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095584-B-C41/ES/EXPLOTACION DE BACTERIAS AMBIENTALES ADAPTADAS A LA ESCASEZ DE AGUA PARA LA INGENIERIA DE BIOPROCESOS EN FASE SOLIDA/es_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095584-B-C42/ES/PROGRAMACION GENETICA DE PSEUDOMONAS PUTIDA PARA UNA EFICACIA CATALITICA SOSTENIDA A TRAVES DE LA ESCALA DE DISPONIBILIDAD DE AGUA/es_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095584-B-C43/ES/EXPLOTACION DE BACTERIAS AMBIENTALES ADAPTADAS A LA ESCASEZ DE AGUA PARA LA INGENIERIA DE BIOPROCESOS EN FASE SOLIDA (SETH)/es_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095584-B-C44/ES/CHASIS XEROTOLERANTES Y APLICACIONES BIOTECNOLOGICAS/es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectNanopore sequencinges_ES
dc.subjectBioprospectinges_ES
dc.subjectMicrobiome analysises_ES
dc.subjectTabernas desertes_ES
dc.titleA round trip to the desert: In situ nanopore sequencing informs targeted bioprospectinges_ES
dc.typeartículoes_ES
dc.identifier.doi10.3389/fmicb.2021.768240-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2021.768240es_ES
dc.identifier.e-issn1664-302X-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.pmid34966365-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
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item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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