Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/261984
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invite to open peer review
Title

A round trip to the desert: In situ nanopore sequencing informs targeted bioprospecting

AuthorsLatorre-Pérez, Adriel; Gimeno-Valero, Helena; Tanner, Kristie; Pascual, Javier; Vilanova, Cristina; Porcar, Manuel CSIC ORCID
KeywordsNanopore sequencing
Bioprospecting
Microbiome analysis
Tabernas desert
Issue Date2021
PublisherFrontiers Media
CitationFrontiers in Microbiology 12: 768240 (2021)
AbstractBioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile laboratory. Results enabled the identification of locations showing the greatest and the least potential, and a second, informed sampling was performed focusing on those sites. After finishing the expedition, a culture collection of 166 strains belonging to 50 different genera was established. Overall, Nanopore and culturing data correlated well, since samples holding a greater potential at the microbiome level also yielded a more interesting set of microbial isolates, whereas samples showing less biodiversity resulted in a reduced (and redundant) set of culturable bacteria. Thus, we anticipate that portable sequencers hold potential as key, easy-to-use tools for in situ-informed bioprospecting strategies.
Publisher version (URL)https://doi.org/10.3389/fmicb.2021.768240
URIhttp://hdl.handle.net/10261/261984
DOI10.3389/fmicb.2021.768240
E-ISSN1664-302X
Appears in Collections:(I2SysBio) Artículos




Files in This Item:
File Description SizeFormat
aroundbio.pdf3 MBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

PubMed Central
Citations

6
checked on Apr 10, 2024

SCOPUSTM   
Citations

8
checked on Apr 13, 2024

WEB OF SCIENCETM
Citations

9
checked on Feb 25, 2024

Page view(s)

186
checked on Apr 13, 2024

Download(s)

234
checked on Apr 13, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Related articles:


This item is licensed under a Creative Commons License Creative Commons