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Título

Two mathematical models for the correction of carbohydrate and protein interference in the determination of uronic acids by the m-hydroxydiphenyl method

AutorMurado García, Miguel Anxo ; Vázquez, José Antonio ; Montemayor, María I. ; López Cabo, Marta ; González Fernández, Pilar
Fecha de publicación2005
EditorPortland Press
CitaciónBiotechnology and Applied Biochemistry 41: 209-216 (2005)
ResumenThe most common method in the routine determination of uronic acids, the m-hydroxydiphenyl reaction, recently adapted to rapid microplate analysis, has as a main inconvenience, in any one of their modalities, interferences due to the frequent presence of proteins and neutral carbohydrates in the samples. Corresponding corrections in the literature are unsatisfactory when applied to complex matrices, and further adaptation to the microplate analysis is not free from additional problems. With particular reference to hyaluronic acid, the interactions between the principal reactants and the interfering materials are studied kinetically under realistic conditions, and simple mathematical models are proposed which satisfactorily describe the experimental results and allow adequate corrections to be made.
Descripción8 pages, 3 figures, 2 tables.
Versión del editorhttp://dx.doi.org/10.1042/BA20040127
URIhttp://hdl.handle.net/10261/26138
DOI10.1042/BA20040127
ISSN0885-4513
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