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Title

Two mathematical models for the correction of carbohydrate and protein interference in the determination of uronic acids by the m-hydroxydiphenyl method

AuthorsMurado García, Miguel Anxo; Vázquez, José Antonio CSIC ORCID; Montemayor, María I. CSIC; López Cabo, Marta CSIC; González Fernández, Pilar CSIC
Issue Date2005
PublisherPortland Press
CitationBiotechnology and Applied Biochemistry 41: 209-216 (2005)
AbstractThe most common method in the routine determination of uronic acids, the m-hydroxydiphenyl reaction, recently adapted to rapid microplate analysis, has as a main inconvenience, in any one of their modalities, interferences due to the frequent presence of proteins and neutral carbohydrates in the samples. Corresponding corrections in the literature are unsatisfactory when applied to complex matrices, and further adaptation to the microplate analysis is not free from additional problems. With particular reference to hyaluronic acid, the interactions between the principal reactants and the interfering materials are studied kinetically under realistic conditions, and simple mathematical models are proposed which satisfactorily describe the experimental results and allow adequate corrections to be made.
Description8 pages, 3 figures, 2 tables.
Publisher version (URL)http://dx.doi.org/10.1042/BA20040127
URIhttp://hdl.handle.net/10261/26138
DOI10.1042/BA20040127
ISSN0885-4513
Appears in Collections:(IIM) Artículos

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