English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/26060
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Chlorophyll c2 monogalactosyldiacylglyceride ester (chl c2-MGDG). A novel marker pigment for Chrysochromulina species (Haptophyta)

AutorZapata, Manuel ; Edvardsen, Bente; Rodríguez, Francisco; Maestro, Miguel Ángel; Garrido, J. L.
Palabras claveChlorophyll c2-galactolipid esters
Chrysochromulina polylepis
Chrysochromulina spp.
Fast atom bombardment-mass spectrometry
HPLC pigment analysis
Marker pigments
Chemotaxonomy
Fecha de publicaciónsep-2001
EditorInter Research
CitaciónMarine Ecology Progress Series 219: 85-98 (2001)
ResumenThe pigment composition of the haptophyte Chrysochromulina polylepis (Strain CCMP 286) was analysed by high-performance liquid chromatography (HPLC) using pyridine-containing mobile phases and polymeric C18, or monomeric C8 columns. The polar chlorophyll (chl) c pigment composition included chl c2 and chl c3 as major fractions, and divinyl protochlorophyllide a (DV pchlide or MgDVP) as a minor component. Several non-polar fluorescent peaks sharing a common chl c-type absorption spectrum were also detected. The main component of these peaks was isolated and characterised by chromatographic behaviour, UV-visible (UV-VIS) and fluorescence spectroscopy. Although spectral properties were similar to a high molecular weight non-polar chl c (1313 Da) recently characterised from Emiliania huxleyi, both chlorophylls showed different chromatographic behaviour. Fast atom bombardment-mass spectrometry (FAB-MS) analysis showed a high mass molecular ion (m/z 1265), and a fragmentation pattern compatible with a molecular structure consisting of a chl c2 pigment linked by an ester bond to the sugar moiety of a monogalactosyl diacylglyceride (MGDG), which included 2 myristic acid (14:0) residues. To study the distribution pattern of the novel pigment, different strains of C. polylepis and other species of the genus Chrysochromulina were analysed. A similar chl c2 ester was also present in 2 C. polylepis strains (K, B11) and in C. aff. polylepis (PLY 200), as well as in C. acantha, C. camella, C. leadbeateri, C. strobilus, C. throndsenii, and Chrysochromulina sp. (CS-410). Three species, C. fragaria, C. hirta (2 strains) and C. kappa, lacked the new chl c, but they contained detectable amounts of other non-polar chl c-like pigments. The feasibility of using chls c and carotenoids as marker pigments to detect Chrysochromulina species in field samples is discussed.
Descripción14 pages, 6 figures, 6 tables.
Versión del editorhttp://dx.doi.org/10.3354/meps219085
URIhttp://hdl.handle.net/10261/26060
DOI10.3354/meps219085
ISSN0171-8630
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
m219p085.pdf476,1 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.