English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/26042
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

In vitro intestinal absorption of carotenoids delivered as molecular inclusion complexes with b-cyclodextrin is not inhibited by high-density lipoproteins

AuthorsFernández García, Elisabet ; Carvajal-Lérida, Irene ; Rincón, Francisco; Ríos Martín, José Julián ; Pérez Gálvez, Antonio
KeywordsCarotenoid
Provitamin A
Assimilation
Beta-cyclodextrin
Brush border membrane vesicles
Lutein
High-density lipoproteins
Membrane protein transporters
Issue Date7-Jul-2010
AbstractThis study analyzed the assimilation efficiency of carotenoids when they are delivered as inclusion complexes with b-cyclodextrin (CyDIC) in water. The in vitro assimilation model used was the brush border membrane vesicles (BBMV) system in which the BBMVs were incubated with CyDIC. Carotenoid suspensions in Tween were used as a reference. Regardless of the form in which the carotenoids were delivered to the BBMV preparation, a higher assimilation efficiency was observed for carotenes than for the xanthophyll lutein. At the highest donor solution concentration, supplying carotenoids in CyDIC produced a significant increase in carotenoid assimilation compared to the corresponding carotenoid suspensions in Tween. The assimilation process using CyDIC takes place by means of a dissociation process in which the carotenoid are released from the b-cyclodextrin to later be assimilated. At the highest concentration of CyDIC in the donor solution, the dissociation equilibrium will be shifted toward the free forms of the complex, thus increasing the amount of carotenoids available for assimilation. In another set of experiments, the effect of high-density lipoproteins as activity inhibitors for the receptors involved in carotenoid assimilation was analyzed. In carotenoid suspensions in Tween, with an inhibitor, a significant decrease in the assimilated quantity compared was observed with values reached without the inhibitor. Lutein presented the most significant decrease (70%). The fact that complete inhibition was not reached suggests that both simple and facilitated diffusion contributes to the assimilation process. When the donor solution composed of CyDIC and inhibitor was added, significant increases were observed in b-carotene and lycopene assimilation for all concentrations, and in lutein for the highest concentration. This effect is due to the exchange between lipoprotein lipid components and the CyDIC, which promotes the dissociation and liberation processes of the carotenoid, which then becomes available for assimilation.
URIhttp://hdl.handle.net/10261/26042
ISSN978-963-9970-04-5
Appears in Collections:(IG) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
PFT_VI_APG.ppt1,68 MBMicrosoft PowerpointView/Open
Show full item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.