Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/258474
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A spectroscopy approach to the study of virus infection in the endophytic fungus Epichloë festucae |
Autor: | Petisco, Cristina CSIC; García Criado, Balbino CSIC; Zabalgogeazcoa, Iñigo ; Vázquez de Aldana, Beatriz R. CSIC ORCID ; García Ciudad, Antonia CSIC | Palabras clave: | Epichloë festucae Festuca rubra fungal endophytes Near-infrared spectroscopy viruses |
Fecha de publicación: | 8-jun-2011 | Editor: | BioMed Central | Citación: | Virology Journal 8: 286 (2011) | Resumen: | Background: In this work we propose a rapid method based on visible and near-infrared (Vis-NIR) spectroscopy to determine the occurrence of double-stranded RNA (dsRNA) viruses in Epichloë festucae strains isolated from Festuca rubra plants. In addition, we examined the incidence of infections by E. festucae in populations of F. rubra collected in natural grasslands of Western Spain. Methods: Vis-NIR spectra (400-2498 nm) from 124 virus-infected and virus-free E. festucae isolates were recorded directly from ground and freeze-dried mycelium. To estimate how well the spectra for uninfected and infected fungal samples could be differentiated, we used partial least-squares discriminant analysis (PLS1-DA) and several data pre-treatments to develop calibration models. Results: Applying the best regression model, obtained with two sampling years and using standard normal variate (SNV) combined with first derivative transformation to a new validating data set (42 samples), we obtained a correct classification for 75% of the uninfected isolates and up to 86% of the infected isolates. Conclusions: The results obtained suggest that Vis-NIR spectroscopy is a promising technology for detection of viral infections in fungal samples when an alternative faster approach is desirable. It provides a tool adequately exact and more time- and cost-saving than the conventional reference analysis. | Versión del editor: | http://dx.doi.org/10.1186/1743-422X-8-286 | URI: | http://hdl.handle.net/10261/258474 | DOI: | 10.1186/1743-422X-8-286 | Identificadores: | doi: 10.1186/1743-422X-8-286 issn: 1743-422X |
Aparece en las colecciones: | (IRNASA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 23-abr-2024
SCOPUSTM
Citations
8
checked on 17-abr-2024
WEB OF SCIENCETM
Citations
8
checked on 28-feb-2024
Page view(s)
45
checked on 24-abr-2024
Download(s)
11
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.