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Título: | The mechanism of the transpersulfuration reaction in a cysteine desulfurase-sulfur acceptor model system |
Autor: | Fernández, Francisco J. CSIC ORCID; Ardá, Ana CSIC ORCID CVN; López-Estepa, Miguel CSIC; Peña-Soler, Esther CSIC; Aranda, Juan; Garces, Fernando CSIC; Quintana, Juan F. CSIC; Round, Adam; Campos-Olivas, Ramón CSIC ORCID; Bruix, M. CSIC ORCID ; Coll, Miquel CSIC ORCID ; Tuñon, Iñaki; Jiménez-Barbero, Jesús CSIC ORCID; Vega, María Cristina CSIC ORCID | Fecha de publicación: | 2-jul-2015 | Citación: | 1as Jornadas Españolas de Biocatálisis (2015) | Resumen: | Escherichia coli CsdA cysteine desulfurase (the sulfur donor) and the CsdE sulfur acceptor are involved in biological sulfur trafficking, in iron-sulfur cluster assembly, and tRNA hypermodification [1] in the model bacterium Escherichia coli. CsdA and CsdE form a stable complex through a polar interface. Although mechanisms for the transfer of a sulfur moiety across protein-protein interfaces have been proposed based on the IscS-IscU and IscS-TusA structures [2,3], the flexibility of the catalytic Cys loops involved has precluded a high resolution view of the active-site geometry and chemical environment responsible to facilitate sulfur transfer. Here, we have used a combination of X-ray crystallography, solution NMR, biophysical and computational chemistry methods to unravel how CsdA provides a specific recognition platform for CsdE and how their complex organizes a composite functional reaction environment. A mechanistic view of sulfur transfer across protein-protein interfaces emerges from the structural analysis of the CSD system. | Descripción: | Trabajo presentado en las 1as Jornadas Españolas de Biocatálisis, celebradas en Madrid (España) del 02 al 03 de julio de 2015. | URI: | http://hdl.handle.net/10261/258145 |
Aparece en las colecciones: | (IQF) Comunicaciones congresos (CIB) Comunicaciones congresos |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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