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Título: | Method for the production of DSRNA |
Autor: | Daròs Arnau, José Antonio CSIC ORCID ; Ortolá Navarro, Beltrán; Cordero, María Teresa | Fecha de publicación: | 19-may-2021 | Citación: | EP3822353 A1 | Resumen: | The present invention relates to an isolated nucleotide acid sequence comprising a) the cDNAs of two strands of a target dsRNA separated by an autocatalytic intron flanked by exon fragments, and b) a plant viroid, wherein element (a) is inserted in the plant viroid sequence. The expression of this nucleotide sequence in a host cell, such as E. coli, along with a tRNA ligase, allows the production of high amounts of dsRNA specific for a target gene. This dsRNA can be used in the interfering RNA technology for silencing gene expression. Additionally, the present invention also relates to a method for producing said dsRNA | URI: | http://hdl.handle.net/10261/257036 |
Aparece en las colecciones: | (IBMCP) Patentes |
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EP3822353A1.pdf | 24,92 MB | Adobe PDF | Visualizar/Abrir |
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