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Título

In vitro culture of Perkinsus atlanticus, a parasite of the carpet shell clam Ruditapes decussatus

AutorOrdás, M. Camino ; Figueras Huerta, Antonio
Palabras clavePerkinsus atlanticus
Clams
Protistan
Protozoa
Parasite
In vitro
Ruditapes decussatus
Fecha de publicación19-jun-1998
EditorInter Research
CitaciónDiseases of Aquatic Organisms 33(2): 129-136 (1998)
ResumenThe ability to mass-culture parasites in vitro facilitates biological, metabolic and morphological research that would otherwise be difficult to accomplish. In vitro continuous culture of the protistan Perkinsus atlanticus from carpet-shell clam was established and variables including culture medium, inoculum size, temperature and salinity, which affect parasite proliferation, were studied. This parasite can adapt to very different culture media and salinity and temperature conditions, and the inoculum density does not affect the final cellular concentration attained in vitro. The morphology of cultured P. atlanticus is described and its optimum in vitro growth conditions were compared with those for P. marinus, a parasite of the eastern oyster Crassostrea virginica. Cryopreservation of cultured P. atlanticus was developed to assure the long-term storage of this parasite.
Descripción8 pages, 8 figures.
Versión del editorhttp://dx.doi.org/10.3354/dao033129
URIhttp://hdl.handle.net/10261/25657
DOI10.3354/dao033129
ISSN0177-5103
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