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Title

Application of high-performance liquid chromatography–tandem mass spectrometry to the identification of biologically active peptides produced by milk fermentation and simulated gastrointestinal digestion

AuthorsHernández-Ledesma, Blanca CSIC ORCID ; Amigo, Lourdes CSIC ORCID ; Ramos González, María Mercedes CSIC ; Recio, Isidra CSIC ORCID
KeywordsTandem mass spectrometry
ACE-inhibitory activity
Bioactive peptides
Fermented milk
Simulated gastrointestinal digestion
Issue Date17-Sep-2004
PublisherElsevier
CitationJournal of Chromatography A 1049(1-2): 107-114 (2004)
AbstractIdentification of biologically active peptides in food matrices is a challenging task in food technology. In the present study, we propose a strategy for the rapid identification of peptides in complex food fractions and targeting of potentially bioactive peptides according to previous studies of activity-structure relationship. A large number of peptides included in the Mr 3000 permeate of a fermented product and its hydrolysate (obtained by simulated gastrointestinal digestion) could be easily identified using HPLC coupled on line to an ion trap mass spectrometer. Three of the identified sequences have previously been described as angiotensin-converting enzyme (ACE) inhibitors. The sequence of some peptides allowed us to anticipate the presence of ACE-inhibitory activity and several peptides were selected to initiate studies on antihypertensive, antioxidant and cytomodulatory activity.
Publisher version (URL)http://dx.doi.org/10.1016/j.chroma.2004.07.025
URIhttp://hdl.handle.net/10261/256512
DOI10.1016/j.chroma.2004.07.025
Identifiersdoi: 10.1016/j.chroma.2004.07.025
issn: 0021-9673
Appears in Collections:(IFI) Artículos
(CIAL) Artículos




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