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Title

Application of mass spectrometry to the characterization and quantification of food-derived bioactive peptides

AuthorsContreras, María del Mar CSIC ORCID; López-Expósito, Iván CSIC; Hernández-Ledesma, Blanca CSIC ORCID ; Ramos González, María Mercedes CSIC ; Recio, Isidra CSIC ORCID
Issue Date1-Jul-2008
PublisherAOAC International
CitationJournal of AOAC International 91(4): 981-994 (2008)
AbstractBiologically active peptides are of particular interest in food science and nutrition because they have been shown to play different physiological roles, including antihypertensive, opioid, antimicrobial, and immunostimulating activities. Because these peptides are generated by protein hydrolysis or fermentation, they can represent only minor constituents in a highly complex matrix and therefore, identification of biologically active peptides in food matrixes is a challenging task in food technology. In this context, mass spectrometry (MS) has developed into a necessary tool to assess quality and safety of food and, more recently, to determine the presence and behavior of functional components such as these bioactive peptides. This review highlights the existing methods based on MS to identify, characterize, and quantify food-derived biologically active peptides, taking into account the different ionization sources used for the analysis of these high-value food components. The quantitative determination of bioactive peptides in food products or biological fluids is also discussed.
Publisher version (URL)https://doi.org/10.1093/jaoac/91.4.981
URIhttp://hdl.handle.net/10261/256367
DOI10.1093/jaoac/91.4.981
Identifiersdoi: 10.1093/jaoac/91.4.981
issn: 1060-3271
e-issn: 1944-7922
Appears in Collections:(IFI) Artículos




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