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Título: | Role of cyclooxygenase 2 (COX-2) in liver mitochondrial function after ischemia-reperfusion injury |
Autor: | Fuertes-Agudo, Marina CSIC ORCID; Cucarella, Carme CSIC ; Brea, Rocío CSIC; Boscá, Lisardo CSIC ORCID CVN ; Martín-Sanz, Paloma CSIC ORCID ; Casado, Marta CSIC ORCID | Fecha de publicación: | oct-2021 | Editor: | Wiley-Blackwell American Association for the Study of Liver Diseases |
Citación: | Hepatology 74 (Supplement1):293A-293A. Meeting Abstract 470 (2021) | Resumen: | Background: Adult hepatocytes fail to induce cyclooxygenase 1 (COX-2) expression, the key enzyme in the synthesis of prostaglandins, regardless of the pro-inflammatory factors used. COX-2 expression is restricted to those situations in which dedifferentiation or proliferation occur. In parallel using mice genetically modified to selectively express human COX-2 (hCOX-2-Tg) in hepatocytes [Protective Role of Hepatocyte Cyclooxygenase-2 Expression Against Liver Ischemia-Reperfusion Injury in Mice. Motiño et al. Hepatology. 2019 Aug;70(2):650-665. https://doi.org/10.1002/hep.30241], we have demonstrated an increased tolerance to ischemia-reperfusion injury (IRI) with an increased functional recovery, a diminished cellular necrosis and less inflammation. It is known that mitochondria have a major role in IRI damage by increasing oxidative stress, decoupling metabolic state, and inducing apoptosis. In this work, we analysed different aspects in order to characterize the impact of COX-2 in mitochondrial function after a 90 min ischemia followed by 4h of reperfusion. Methods: We performed high-resolution respirometry with isolated mitochondria and liver homogenates from hCOX-2-Tg, as well as we analyzed the expression of different components of the electron transport chain supercomplexes, mitochondria morphology by transfer electron microscopy, fusion/fission events and gene or protein expression involved on mitochondria function. Results: Our overall results suggest that hCOX-2-Tg mice-derived mitochondria are more active than wild-type-derived mitochondria, apparently because of a higher contribution from fatty acids metabolism and complex I subtrates. Conclusion: These data suggest a new link between COX-2 and mitochondria, which might contribute to the protective effects of COX-2 against IRI. | Descripción: | Publicación en la revista Hepatology, de los abstracts de los pósteres presentados al: Liver Meeting AASLD 2021: American Association fort eh Study of Liver Diseases. (Anaheim, CA, USA and virtual), 12-15 de novembre 2021 Abstract: 470, pag. 293A | Versión del editor: | https://doi.org/10.1002/hep.32188 | URI: | http://hdl.handle.net/10261/255305 | DOI: | 10.1002/hep.32188 | ISSN: | 0270-9139 | E-ISSN: | 1527-3350 | Referencias: | Fuertes-Agudo, Marina; Cucarella, Carme CSIC; Brea Contreras, Rocio; Boscá, Lisardo; Martín-Sanz, Paloma; Casado, Marta Role of cyclooxygenase 2 (COX-2) in liver mitochondrial function after ischemia-reperfusion injury. (Póster) Liver Meeting AASLD 2021: American Association fort eh Study of Liver Diseases. (Anaheim, CA, USA and virtual), 12-15 de novembre 2021 Abstract: 470, pag. 293A http://hdl.handle.net/10261/255551 |
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