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An epizootic in farmed, market-sized rainbow trout in Spain caused by a strain of Carnobactenum piscicola of unusual virulence

AutorToranzo, A. E.; Romalde, J. L.; Núñez, S.; Figueras Huerta, Antonio ; Barja, J. L.
Palabras claveCarnobacterium piscicola
Rainbow trout
Fecha de publicación1993
EditorInter Research
CitaciónDiseases of Aquatic Organisms 17: 87-99 (1993)
ResumenWe report here the first description In Spain of a Carnobacterium strain causing important mortalities in market-size rainbow trout Oncorhynchus mykiss. Relevant clinical signs in affected fish were a pronounced bilateral exophthalmia withp periocular hemorrhages, accumnulation of ascitic fluid, and hemorrhages in the liver, swimbladder muscle, and intestine. Taxonomic studies conducted in comparison with reference strains indicated that the present isolate (PT-31) was Carnobacterium piscicola. Agglutination assays demonstrated that this isolate was not serologically related to the reference strains. In addition, an analysis of the surface proteins revealed that different patterns occurred among the C. piscicola isolates. Although the immunoblotting assays supported the antigenic heterogeneity within this species, all strains did share 2 major antigenic proteins of 30 and 57 kDa. Interestingly, this immunoreactive 57 kDa protein is also produced by Renibacterium salmoninarum and Corynebacterium aquaticum. Regardless of the challenge method (injection or water-borne) isolate PT-31 proved to be hlghly pathogenic for rainbow trout (LD50 < 5-6 X 104 cells), and the moribund fish displayed the external and internal signs observed in the natural dlsease. The inoculated strain could be recovered from various organs of both dead and surviving fish. The extracellular products (ECP) of strain PT-31 contained exotoxins lethal for rainbow trout ( LD50= 4.5 kg protein g-' fish). However, the toxic ECP lacked enzymatic, cytotoxic and hemolytic activities when tested in vitro. Histopathological examination of naturally and experimentally infected trout showed extensive and/or intenslve lesions in most organs. The most marked tissue damage (acute hemorrhages and necrosis) was observed in eyes, kidney, liver, spleen, pancreas, and muscle. These findings should alert fish farmers to the existence of C. piscicola strains with a pathogenic capability higher than that previously recorded.
Descripción13 pages, 3 figures, 5 tables.
Versión del editorhttp://www.int-res.com/abstracts/dao/v17/
ISSN0177-5103 (Print)
1616-1580 (Online)
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