English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/25451
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

AutorMartínez, Miguel Ángel; Verdaguer, Núria ; Mateu, Mauricio G.; Domingo, Esteban
Fecha de publicación24-jun-1997
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences of the USA 94(13): 6798–6802 (1997)
ResumenAphthoviruses use a conserved Arg-Gly-Asp triplet for attachment to host cells and this motif is believed to be essential for virus viability. Here we report that this triplet—which is also a widespread motif involved in cell-to-cell adhesion—can become dispensable upon short-term evolution of the virus harboring it. Foot-and-mouth disease virus (FMDV), which was multiply passaged in cell culture, showed an altered repertoire of antigenic variants resistant to a neutralizing monoclonal antibody. The altered repertoire includes variants with substitutions at the Arg-Gly-Asp motif. Mutants lacking this sequence replicated normally in cell culture and were indistinguishable from the parental virus. Studies with individual FMDV clones indicate that amino acid replacements on the capsid surface located around the loop harboring the Arg-Gly-Asp triplet may mediate in the dispensability of this motif. The results show that FMDV quasispecies evolving in a constant biological environment have the capability of rendering totally dispensable a receptor recognition motif previously invariant, and to ensure an alternative pathway for normal viral replication. Thus, variability of highly conserved motifs, even those that viruses have adapted from functional cellular motifs, can contribute to phenotypic flexibility of RNA viruses in nature.
Descripción5 pages, 3 figures, 3 tables.-- PMID: 9192645 [PubMed].-- PMCID: PMC21238.
Versión del editorhttp://www.pnas.org/content/94/13/6798.long
URIhttp://hdl.handle.net/10261/25451
ISSN0027-8424 (Print)
1091-6490 (Online)
Aparece en las colecciones: (IDAEA) Artículos
(CID) Artículos
(IBMB) Artículos
(CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Martinez_Miguel_et_al.pdf265,56 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.