English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/25363
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Campo DC Valor Lengua/Idioma
dc.contributor.authorNovoa, Beatriz-
dc.contributor.authorBlake, S.-
dc.contributor.authorNicholson, B. L.-
dc.contributor.authorFigueras Huerta, Antonio-
dc.date.accessioned2010-06-17T09:35:33Z-
dc.date.available2010-06-17T09:35:33Z-
dc.date.issued1995-08-
dc.identifier.citationApplied and Environmental Microbiology 61(8): 2925-2929 (1995)en_US
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/25363-
dc.description5 pages, 2 figures, 5 tables.en_US
dc.description.abstractThe current classification of aquatic birnaviruses is based on seroneutralization assays with polyclonal antibodies. In this study a comparison of several procedures used for serotyping aquatic birnaviruses was made with 10 virus strains (4 reference strains from salmonids and 6 birnaviruses isolated from turbot [Scophthalmus maximus]). The relationships among the birnavirus strains were studied by seroneutralization assay with polyclonal antibodies and by immunodot assay with both polyclonal and monoclonal antibodies. The results were compared with a presumptive classification obtained from analysis of restriction enzyme patterns of cDNA products obtained by PCR amplification. No correlation was found among the results obtained by the different procedures. The seroneutralization and the immunodot assays with polyclonal antibodies were not useful in classifying these birnaviruses strains; however, patterns of reaction with monoclonal antibodies emphasized the individuality of the strains, particularly in the case of two strains (231 and 460) whose patterns did not correspond to established serotypes. The application of PCR and restriction enzyme analysis is a promising system for approaching the classification of this viral group on the basis of genomic differences and similarities. The variable results obtained in this comparison lead us to think that the current classification of aquatic birnavirus may not be the most accurate and there is a need for modification incorporating recent isolates, not only from salmonid species but also from marine fish.en_US
dc.description.sponsorshipThis work was supported by grant AGF93-0769-C02-02 from the Comisión Interministerial de Ciencia y Tecnología. Beatriz Novoa acknowledges the Ministerio de Educación y Ciencia (Spain) for a research fellowship.en_US
dc.format.extent391208 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsclosedAccessen_US
dc.titleComparison of different procedures for serotyping aquatic birnavirusen_US
dc.typeArtículoen_US
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://aem.asm.org/cgi/content/abstract/61/8/2925en_US
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.