English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/25338
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Characterization of a birnavirus isolated from diseased turbot cultured in Spain

AutorNovoa, Beatriz ; Figueras Huerta, Antonio; Puentes, C. F.; Ledo, A.; Toranzo, A. E.
Fecha de publicación29-abr-1993
EditorInter Research
CitaciónDiseases of Aquatic Organisms 15: 163-169 (1993)
ResumenDuring 1989, light but persistent mortalities were detected in a turbot Scophthalmus maximus L. farm in Galicia (northwestern Spain) and a virus with the characteristics of a birnavirus was isolated. The purpose of this study was to characterize the viral agent and determine the susceptibility of turbot to this virus. Electron microscopic examination revealed that the particles were isometric, hexagonal and unenveloped with an average diameter of 58 to 60 nm. The molecular weights of the RNA segments were 1.9 and 2.0 x 10(up to 6) daltons. The cells most susceptible to the turbot isolate were the CHSE-214, FHM and RTG-2 lines and the optimal temperature range for its replication was 15 to 2OºC. The RNA and polypeptide electropherotypes show that this virus resembles the Ab serotype of infectious pancreatic necrosis virus (IPNV); however, it differs in that it replicates in the FHM cell line and is not neutralized by antisera to the classical serotypes of IPNV. Infectivity trials conducted in turbot of dlfferent sizes indicated that the virus produced mortality only in small fish (2 g), although the larger fish (30 g) harbored the virus for at least 35 d. Fish inoculated with this isolate showed no pancreatic necrosis although necrosis of the hematopoietic elements of the kidney and spleen was detected.
Descripción7 pages, 5 figures, 2 tables.
Versión del editorhttp://www.int-res.com/abstracts/dao/v15/
URIhttp://hdl.handle.net/10261/25338
ISSN0177-5103
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
d015p163.pdf2,09 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.