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Título: | Modification of 13-hydroperoxide lyase expression in olive affects plant growth and results in altered volatile profile |
Autor: | Cerezo, Sergio; Hernández, María Luisa; Palomo-Ríos, Elena; Gouffi, Naima; García-Vico, Lourdes CSIC; Sicardo, María Dolores; Sanz, Carlos CSIC ORCID ; Mercado, José A.; Pliego-Alfaro, Fernando; Martínez-Rivas, José Manuel CSIC ORCID | Palabras clave: | Gene silencing HPL Olea europaea Overexpression Transgenic plants Volatiles |
Fecha de publicación: | 7-oct-2021 | Editor: | Elsevier | Citación: | Plant science 313: 111083 (2021) | Resumen: | The C6 aldehydes, alcohols, and the corresponding esters are the most important compounds of virgin olive oil aroma. These C6 volatile compounds are synthesized via the 13-hydroperoxide lyase (13-HPL) branch of the lipoxygenase pathway. In this investigation, a functional analysis of the olive (Olea europaea L.) 13-HPL gene by its overexpression and silencing in olive transgenic lines was carried out. With this aim, sense and RNAi constructs of the olive 13-HPL gene were generated and used for the transformation of embryogenic olive cultures. Leaves from overexpressing lines showed a slight increase in 13-HPL gene expression, whereas RNAi lines exhibited a strong decrease in their transcript levels. Quantification of 13-HPL activity in two overexpressing and two RNAi lines showed a positive correlation with levels of transcripts. Interestingly, RNAi lines showed a high decrease in the content of C6 volatiles linked to a strong increase of C5 volatile compounds, altering the volatile profile in the leaves. In addition, the silencing of the 13-HPL gene severely affected plant growth and development. This investigation demonstrates the role of the 13-HPL gene in the biogenesis of olive volatile compounds and constitutes a functional genomics study in olive related to virgin olive oil quality. | Descripción: | 10 Páginas.-- 5 Figuras.-- 2 Tablas | Versión del editor: | http://dx.doi.org/10.1016/j.plantsci.2021.111083 | URI: | http://hdl.handle.net/10261/253072 | ISSN: | 0168-9452 |
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Plant_Science_2021_V313_111083.pdf | Artículo principal | 1,91 MB | Adobe PDF | Visualizar/Abrir |
Plant Science_2021_V313_111083_Material suplementario.pdf | Material suplementario | 126,16 kB | Adobe PDF | Visualizar/Abrir |
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