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Título

The large nonsymbiotic plasmid pRmeGR4a of Rhizobium meliloti GR4 encodes a protein involved in replication that has homology with RepC proteins of Agrobacterium plasmids

AutorMercado-Blanco, Jesús ; Olivares Pascual, José
Fecha de publicaciónjul-1994
EditorAcademic Press
CitaciónPlasmid 32: 75-79 (1994)
ResumenThe large plasmid, pRmeGR4a, of Rhizobium meliloti GR4 is a nonsymbiotic, self-transmissible replicon that shows a high degree of stability. Plasmid replication and stabilization functions are located on a 4.8-kb PstI fragment. Analysis of the nucleotide sequence of this DNA region revealed the presence of six open reading frames (ORFs) with coding capabilities; all are transcribed in the same direction. Only one of the ORFs (ORF3) seems to be essential for replication. Its predicted protein sequence shows homology with RepC, a protein that has been suggested to be involved in the replication of Agrobacterium Ri and Ti plasmids.
Descripción5 pages, 2 figures.
Versión del editorhttp://dx.doi.org/10.1006/plas.1994.1046
URIhttp://hdl.handle.net/10261/25224
DOI10.1006/plas.1994.1046
ISSN0147-619X
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