English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/25193
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 25 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Conformationally rigid nucleoside probes help understand the role of sugar pucker and nucleobase orientation in the thrombin-binding aptamer

AutorSaneyoshi, Hisao; Mazzini, Stefania; Aviñó, Anna; Portella, Guillem; González, Carlos ; Orozco, Modesto; Márquez, Víctor E.; Eritja Casadellà, Ramón
Palabras claveThrombin-Binding Aptamer (TBA)
NMR spectra
Fecha de publicación20-jul-2009
EditorOxford University Press
CitaciónNucleic Acids Research 37(17): 5589–5601 (2009)
ResumenModified thrombin-binding aptamers carrying 2′-deoxyguanine (dG) residues with locked North- or South-bicyclo[3.1.0]hexane pseudosugars were synthesized. Individual 2′-deoxyguanosines at positions dG5, dG10, dG14 and dG15 of the aptamer were replaced by these analogues where the North/anti and South/syn conformational states were confined. It was found that the global structure of the DNA aptamer was, for the most part, very accommodating. The substitution at positions 5, 10 and 14 with a locked South/syn-dG nucleoside produced aptamers with the same stability and global structure as the innate, unmodified one. Replacing position 15 with the same South/syn-dG nucleoside induced a strong destabilization of the aptamer, while the antipodal North/anti-dG nucleoside was less destabilizing. Remarkably, the insertion of a North/anti-dG nucleoside at position 14, where both pseudosugar conformation and glycosyl torsion angle are opposite with respect to the native structure, led to the complete disruption of the G-tetraplex structure as detected by NMR and confirmed by extensive molecular dynamics simulations. We conclude that conformationally locked bicyclo[3.1.0]hexane nucleosides appear to be excellent tools for studying the role of key conformational parameters that are critical for the formation of a stable, antiparallel G-tetrad DNA structures.
Descripción13 pages, 8 figures, 2 tables.-- PMID: 19620215 [PubMed].-- PMCID: PMC2761269.-- Printed version published Sep 2009.
Versión del editorhttp://dx.doi.org/10.1093/nar/gkp598
URIhttp://hdl.handle.net/10261/25193
DOI10.1093/nar/gkp598
ISSN0305-1048 (Print)
1362-4962 (Online)
Aparece en las colecciones: (IQAC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Saneyoshi_Hisao_et_al.pdf17,59 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.