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Título

Localization of manganese superoxide dismutase promoter activity in nodules of Lotus japonicus

AutorRubio Luna, María Carmen ; Becana Ausejo, Manuel
Fecha de publicaciónjun-2010
EditorSociedad Española de Fijación de Nitrógeno
CitaciónRubio M.C., Becana, M., Localization of manganese superoxide dismutase promoter. En: Biological Nitrogen Fixation and Plant-Associated Microorganisms. XIII SEFIN. XIII National Meeting of the Spanish Society of Nitrogen Fixation; II Portuguese-Spanish Congress on Nitrogen Fixation. Zaragoza (Spain), 15-18 June 2010 . S4-19, p. 217-218.
ResumenWe have performed a preliminary analysis of the promoter activity of the manganese superoxide dismutase gene (LjMnSOD) during nodule development in the model legume Lotus japonicus. The putative promoter region was amplified by PCR and cloned into a binary vector containing GUS (β-glucuronidase) as the reporter gene. Transgenic roots and nodules were produced by infection with Agrobacterium rhizogenes LBA1334 using the ‘hairy roots’ system. Histochemical analysis of GUS activity showed that the LjMnSOD promoter is active in the vascular tissue of the nodule, particularly at the base, where nodules are connected to the subtending root. Promoter activity increased during nodule development. These data suggest an important role of MnSOD in the nodule vascular tissue, which could be related to the high respiratory activity of the vascular bundle cells.
Descripción2 Pag., 1 Fig.
URIhttp://hdl.handle.net/10261/25179
ReferenciasD.L. Hu 212/2010
Aparece en las colecciones: (EEAD) Comunicaciones congresos
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