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Título: | HuConTest: Testing Human Contamination in Great Ape Samples |
Autor: | Kuhlwilm, Martin CSIC ORCID; Fontsere, Claudia CSIC ORCID; Han, Sojung CSIC ORCID; Alvarez-Estape, Marina CSIC ORCID; Marqués-Bonet, Tomàs CSIC ORCID | Palabras clave: | Contamination Nonhuman primates Next-generation sequencing Fecal DNA Ancient DNA |
Fecha de publicación: | jul-2021 | Editor: | Oxford University Press | Citación: | Genome Biology and Evolution 13(6): evab117 (2021) | Resumen: | Modern human contamination is a common problem in ancient DNA studies. We provide evidence that this issue is also present in studies in great apes, which are our closest living relatives, for example in noninvasive samples. Here, we present a simple method to detect human contamination in short-read sequencing data from different species: HuConTest. We demonstrate its feasibility using blood and tissue samples from these species. This test is particularly useful for more complex samples (such as museum and noninvasive samples) which have smaller amounts of endogenous DNA, as we show here. | Versión del editor: | https://doi.org/10.1093/gbe/evab117 | URI: | http://hdl.handle.net/10261/251184 | DOI: | 10.1093/gbe/evab117 | E-ISSN: | 1759-6653 |
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HuConTest.pdf | 674,02 kB | Adobe PDF | Visualizar/Abrir |
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