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Título

Recovery of proteolytic and collagenolytic activities from viscera by-products of Rayfish (Raja clavata)

AutorMurado García, Miguel Anxo ; González Fernández, Pilar ; Vázquez, José Antonio
Palabras claveProteolytic and collagenolytic activity
By-products upgrading
Rayfish viscera wastes
PH and temperature effects
Mathematical modelling
Fecha de publicación15-dic-2009
EditorMultidisciplinary Digital Publishing Institute
CitaciónMarine Drugs 7(4): 803-815 (2009)
ResumenThe aim of this work was to study the recovery of proteolytic and collagenolytic activities from rayfish (Raja clavata) viscera wastes. Initially, different parts of the gastrointestinal tract by-products (stomach, duodenum section including pancreas, final intestine) were evaluated. The extracts from proximal intestine yielded the highest values of both enzymatic activities. Optimal conditions for protease activity quantification were established at pH = 6, T = 40 °C and incubation time ≤20 min. The mathematical equation used to model the joint effect of pH and temperature led to maximum activity at pH = 8.66 and 59.4 °C, respectively. Overcooled acetone was found to be best option for recovery of enzymatic activities in comparison with ethanol, PEG-4000, ammonium sulphate and ultrafiltration system. Finally, a simple and systematic protocol of partial purification and total recovery of proteases and collagenases was defined.
Descripción13 pages, 5 figures, 3 tables.
Versión del editorhttp://dx.doi.org/10.3390/md7040803
URIhttp://hdl.handle.net/10261/25105
DOI10.3390/md7040803
ISSN1660-3397
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