Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/250707
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | In Vivo Binding of Recombination Proteins to Non-DSB DNA Lesions and to Replication Forks |
Autor: | González-Prieto, Román CSIC ORCID; Cabello-Lobato, María J. CSIC; Prado, Félix CSIC ORCID | Palabras clave: | Chromatin endogenous cleavage DNA binding proteins Non-double-strand break Rad52 Replication intermediates Rad51 Replication fork 2D DNA gel electrophoresis |
Fecha de publicación: | 2021 | Editor: | Humana Press Springer Nature |
Citación: | Homologous Recombination. Methods and Protocols: 447-458 (2021) | Serie: | Methods in Molecular Biology 2153 |
Resumen: | Homologous recombination (HR) has been extensively studied in response to DNA double-strand breaks (DSBs). In contrast, much less is known about how HR deals with DNA lesions other than DSBs (e.g., at single-stranded DNA) and replication forks, despite the fact that these DNA structures are associated with most spontaneous recombination events. A major handicap for studying the role of HR at non-DSB DNA lesions and replication forks is the difficulty of discriminating whether a recombination protein is associated with the non-DSB lesion per se or rather with a DSB generated during their processing. Here, we describe a method to follow the in vivo binding of recombination proteins to non-DSB DNA lesions and replication forks. This approach is based on the cleavage and subsequent electrophoretic analysis of the target DNA by the recombination protein fused to the micrococcal nuclease. | Versión del editor: | https://doi.org/10.1007/978-1-0716-0644-5_31 | URI: | http://hdl.handle.net/10261/250707 | DOI: | 10.1007/978-1-0716-0644-5_31 | ISBN: | 978-1-0716-0643-8 | ISSN: | 1064-3745 | E-ISSN: | 1940-6029 |
Aparece en las colecciones: | (CABIMER) Libros y partes de libros |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
75
checked on 27-mar-2024
Download(s)
9
checked on 27-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.