Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/250654
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Green microsaponification-based method for gas chromatography determination of sterol and squalene in cyanobacterial biomass |
Autor: | Bittencourt Fagundes, Mariane; Álvarez-Rivera, Gerardo CSIC ORCID; Guidetti Vendruscolo, Raquel; Voss, Mônica; Arrojo da Silva, Patricia; Smanioto Barin, Juliano; Jacob-Lopes, Eduardo; Queiroz Zepka, Leila; Wagner, Roger | Fecha de publicación: | 2021 | Editor: | Elsevier | Citación: | Talanta 224: 121793 (2021) | Resumen: | Sterol analysis of complex matrices can be very laborious. To minimize the existing drawbacks, a new micro-method of sterols and squalene determination in cyanobacteria was developed and applied to monitor their production of Phormidium autumnale cultured heterotrophically. Sample extraction/saponification and GC analysis of the target compounds were optimized separately using Plackett-Burman design (PB) followed by a central composite rotational design (CCRD). The most influential variables were identified to maximize compound recovery. Chloroform presented the highest capability to extract all target compounds with a horizontal shaker table (HST) for homogenization in the saponification step. For the pretreatment, a small amount of chloroform was used for 90 min at 50 °C and 6 min for the saponification time. The sample introduction in the GC injector was studied by evaluating pressure and injector temperature. High response for sterols and squalene were obtained between 19 and 23 psi and at 310 °C of injection temperature. The new method was able to determine different sterol concentrations: 0.2–0.6 mg kg−1 of squalene, 5–18 mg kg−1 of stigmasterol, 6 mg kg−1 of cholesterol, and 3 mg kg−1 of β-sitosterol, showing high analytical performance and fulfilling all steps, thus proving to be a promising technique. | Versión del editor: | https://doi.org/10.1016/j.talanta.2020.121793 | URI: | http://hdl.handle.net/10261/250654 | DOI: | 10.1016/j.talanta.2020.121793 | ISSN: | 0039-9140 |
Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
10
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
5
checked on 21-feb-2024
Page view(s)
73
checked on 23-abr-2024
Download(s)
11
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.