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Título: | Monitoring Emergence of the SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19) |
Autor: | Carcereny, Albert; Martínez-Velázquez, Adán; Bosch, Albert; Allende, Ana CSIC ORCID ; Truchado, Pilar CSIC ORCID ; Cascales, Jenifer; Romalde, Jesús L.; Lois, Marta; Polo, David; Sánchez Moragas, Gloria CSIC ORCID; Pérez-Cataluña, Alba CSIC ORCID; Díaz-Reolid, Azahara CSIC; Antón, Andrés; Gregori, Josep; García-Cehic, Damir; Quer, Josep; Palau, Margarita; González Ruano, Cristina; Pintó, Rosa María; Guix, Susana | Palabras clave: | SARS-CoV-2 COVID-19 B.1.1.7 variant Wastewater-based epidemiology (WBE) RT-qPCR NGS |
Fecha de publicación: | 16-ago-2021 | Editor: | American Chemical Society | Citación: | Environmental Science and Technology - Columbus 55(17): 11756–11766 (2021) | Resumen: | Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic. | Versión del editor: | https://doi.org/10.1021/acs.est.1c03589 | URI: | http://hdl.handle.net/10261/250551 | DOI: | 10.1021/acs.est.1c03589 | ISSN: | 0013-936X | E-ISSN: | 1520-5851 |
Aparece en las colecciones: | (CEBAS) Artículos (IATA) Artículos (PTI Salud Global) Colección Especial COVID-19 |
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