Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/248949
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Identification of a novel microRNA (miRNA) from rice that targets an alternatively spliced transcript of the Nramp6 (Natural resistance-associated macrophage protein 6) gene involved in pathogen resistance |
Autor: | Campo, Sonia CSIC ORCID; Peris-Peris, Cristina CSIC; Siré, Christelle; Moreno, Ana Beatriz CSIC ORCID; Donaire, Livia CSIC ORCID ; Zytnicki, Matthias; Notredame, Cedric; Llave, César CSIC ORCID ; San Segundo, Blanca CSIC ORCID | Fecha de publicación: | 2013 | Editor: | Wiley-VCH | Citación: | New Phytologist 199(1): 212-227 (2013) | Resumen: | Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high-throughput sequencing for the identification of elicitor-responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa-miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance-associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA-mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity. | Versión del editor: | https://doi.org/10.1111/nph.12292 | URI: | http://hdl.handle.net/10261/248949 | DOI: | 10.1111/nph.12292 | ISSN: | 0028-646X |
Aparece en las colecciones: | (CRAG) Artículos (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
203
checked on 19-abr-2024
WEB OF SCIENCETM
Citations
168
checked on 26-feb-2024
Page view(s)
69
checked on 23-abr-2024
Download(s)
11
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.