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Título

Apolipoprotein E genotyping method by real time PCR, a fast and cost-effective alternative to the TaqMan and FRET assays

AutorCalero-Rueda, Olga; Hortigüela, Rafael; Bullido, María Jesús; Calero, Miguel
Palabras claveApolipoprotein E gene (APOE)
FRET, Fluorescent Resonance Energy
Fecha de publicación15-oct-2009
EditorElsevier
CitaciónJ Neurosci Methods.183(2):238-40 (2009)
ResumenThe apolipoprotein E gene (APOE) polymorphism genotyping has an allegedly important predictive value for coronary heart disorders and Alzheimer’s disease. We developed a simple, fast, cost-effective and suited for high-throughput protocol for determining APOE genotypes by Real Time PCR monitored by SYBR® Green. The method is based on differential amplification by allele-specific primers. These primers have variations in their 3 -end nucleotides such that are specific for one of the two variants in each polymorphic position. By this protocol,we obtained a 100% concordance with the APOE genotypes determined by sequencing analysis. The main advantages of this method are its relative simplicity and the reduced cost compared to other methodologies, such as the TaqMan® and FRET assays.
Versión del editorhttp://dx.doi.org/10.1016/j.jneumeth.2009.06.033
URIhttp://hdl.handle.net/10261/24890
DOI10.1016/j.jneumeth.2009.06.033
ISSN0165-0270
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