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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Chiloeches, Antonio | - |
dc.contributor.author | Sánchez-Pacheco, Aurora | - |
dc.contributor.author | Gil-Araujo, Beatriz | - |
dc.contributor.author | Aranda, Ana | - |
dc.contributor.author | Lasa, Marina | - |
dc.date.accessioned | 2010-05-28T12:45:29Z | - |
dc.date.available | 2010-05-28T12:45:29Z | - |
dc.date.issued | 2008-11 | - |
dc.identifier.citation | Molecular Endocrinology 22(11): 2466-2480 (2008) | en_US |
dc.identifier.issn | 0888-8809 | - |
dc.identifier.uri | http://hdl.handle.net/10261/24848 | - |
dc.description | 14 pages, 5 figures. | en_US |
dc.description.abstract | Thyroid hormone (T3) plays a crucial role in processes such as cell proliferation and differentiation, whereas its implication on cellular apoptosis has not been well documented. Here we examined the effect of T3 on the apoptosis of GH4C1 pituitary cells and the mechanisms underlying this effect. We show that T3 produced a significant increase in apoptosis in serum-depleted conditions. This effect was accompanied by a decrease in nuclear factor-kappaB (NF-kappaB)-dependent transcription, IkappaBalpha phosphorylation, translocation of p65/NF-kappaB to the nucleus, phosphorylation, and transactivation. Moreover, these effects were correlated with a T3-induced decrease in the expression of antiapoptotic gene products, such as members of the inhibitor of apoptosis protein and Bcl-2 families. On the other hand, ERK but not c-Jun N-terminal kinase or MAPK p38, was activated upon exposure to T3, and inhibition of ERK alone abrogated T3-mediated apoptosis. In addition, T3 increased the expression of the MAPK phosphatase, dual specificity phosphatase 1 (DUSP1), in an ERK-dependent manner. Interestingly, the suppression of DUSP1 expression abrogated T3-induced inhibition of NF-kappaB-dependent transcription and p65/NF-kappaB translocation to the nucleus, as well as T3-mediated apoptosis. Overall, our results indicate that T3 induces apoptosis in rat pituitary tumor cells by down-regulating NF-kappaB activity through a mechanism dependent on the ERK/DUSP1 pathway. | en_US |
dc.description.sponsorship | This work was supported by grants from the Fundación Mutua Madrileña (2005X0615), from Fondo de Investigaciones Sanitarias (PI070832), from Ministerio de Educación y Ciencia (BFU2004 3465), from Fondo de Investigaciones Sanitarias (RD06/0020/0036), and the European grant CRESCENDO (FP-018652). A.S.-P. and M.L. are recipients of grants from the Spanish MEC (“Ramón y Cajal” Program). | en_US |
dc.format.extent | 1331617 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Endocrine Society | en_US |
dc.rights | openAccess | en_US |
dc.title | Thyroid hormone-mediated activation of the ERK/dual specificity phosphatase 1 pathway augments the apoptosis of GH4C1 cells by down-regulating nuclear factor-κB activity | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1210/me.2008-0107 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1210/me.2008-0107 | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Thyroid hormone-mediated.pdf | 1,3 MB | Adobe PDF | Visualizar/Abrir |
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