English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/248251
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Circadian waves of transcriptional repression shape PIF-regulated photoperiod-responsive growth in Arabidopsis

AuthorsMartín, Guiomar; Rovira Feixa, Arnau; Veciana, Nil; Soy, Judit; Toledo-Ortiz, Gabriela CSIC ORCID; Gommers, Charlotte M. M.; Boix, Marc; Henriques, Rossana; Minguet, Eugenio G.; Alabadí, David CSIC ORCID ; Halliday, Karen J.; Leivar, Pablo CSIC ORCID; Monte, Elena CSIC ORCID
KeywordsPlant diurnal growth
Hypocotyl elongation
Gating of plant responses
Phytochrome-interacting factors PIFs
Circadian clock
Pseudo-response regulators PRRs
Issue Date2018
CitationCurrent Biology 28(2): 311-318.e5 (2018)
AbstractPlants coordinate their growth and development with the environment through integration of circadian clock and photosensory pathways. In Arabidopsis thaliana, rhythmic hypocotyl elongation in short days (SD) is enhanced at dawn by the basic-helix-loop-helix (bHLH) transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) directly inducing expression of growth-related genes [1, 2, 3, 4, 5, 6]. PIFs accumulate progressively during the night and are targeted for degradation by active phytochromes in the light, when growth is reduced. Although PIF proteins are also detected during the day hours [7, 8, 9, 10], their growth-promoting activity is inhibited through unknown mechanisms. Recently, the core clock components and transcriptional repressors PSEUDO-RESPONSE REGULATORS PRR9/7/5 [11, 12], negative regulators of hypocotyl elongation [13, 14], were described to associate to G boxes [15], the DNA motifs recognized by the PIFs [16, 17], suggesting that PRR and PIF function might converge antagonistically to regulate growth. Here we report that PRR9/7/5 and PIFs physically interact and bind to the same promoter region of pre-dawn-phased, growth-related genes, and we identify the transcription factor CDF5 [18, 19] as target of this interplay. In SD, CDF5 expression is sequentially repressed from morning to dusk by PRRs and induced pre-dawn by PIFs. Consequently, CDF5 accumulates specifically at dawn, when it induces cell elongation. Our findings provide a framework for recent TIMING OF CAB EXPRESSION 1 (TOC1/PRR1) data [5, 20] and reveal that the long described circadian morning-to-midnight waves of the PRR transcriptional repressors (PRR9, PRR7, PRR5, and TOC1) [21] jointly gate PIF activity to dawn to prevent overgrowth through sequential regulation of common PIF-PRR target genes such as CDF5.
Publisher version (URL)https://doi.org/10.1016/j.cub.2017.12.021
Appears in Collections:(IBMCP) Artículos
(CRAG) Artículos
Files in This Item:
File Description SizeFormat 
circarabi.pdf1,51 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.