Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/248060
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in Catharanthus roseus |
Autor: | Guirimand, Grégory; Guihur, Anthony; Phillips, Michael CSIC ORCID; Oudin, Audrey; Glévarec, Gaëlle; Melin, Céline; Papon, Nicolas; Clastre, Marc; St-Pierre, Benoit; Rodriguez-Concepcion, Manuel CSIC ORCID ; Burlat, Vincent; Courdavault, Vincent | Fecha de publicación: | 2012 | Editor: | Springer Nature | Citación: | Plant Molecular Biology 79: 443–459 (2012) | Resumen: | Isopentenyl diphosphate isomerases (IDI) catalyze the interconversion of the two isoprenoid universal C5 units, isopentenyl diphosphate and dimethylally diphosphate, to allow the biosynthesis of the large variety of isoprenoids including both primary and specialized metabolites. This isomerisation is usually performed by two distinct IDI isoforms located either in plastids/peroxisomes or mitochondria/peroxisomes as recently established in Arabidopsis thaliana mainly accumulating primary isoprenoids. By contrast, almost nothing is known in plants accumulating specialized isoprenoids. Here we report the cloning and functional validation of an IDI encoding cDNA (CrIDI1) from Catharanthus roseus that produces high amount of monoterpenoid indole alkaloids. The corresponding gene is expressed in all organs including roots, flowers and young leaves where transcripts have been detected in internal phloem parenchyma and epidermis. The CrIDI1 gene also produces long and short transcripts giving rise to corresponding proteins with and without a N-terminal transit peptide (TP), respectively. Expression of green fluorescent protein fusions revealed that the long isoform is targeted to both plastids and mitochondria with an apparent similar efficiency. Deletion/fusion experiments established that the first 18-residues of the N-terminal TP are solely responsible of the mitochondria targeting while the entire 77-residue long TP is needed for an additional plastid localization. The short isoform is targeted to peroxisomes in agreement with the presence of peroxisome targeting sequence at its C-terminal end. This complex plastid/mitochondria/peroxisomes triple targeting occurring in C. roseus producing specialized isoprenoid secondary metabolites is somehow different from the situation observed in A. thaliana mainly producing housekeeping isoprenoid metabolites. | Versión del editor: | https://doi.org/10.1007/s11103-012-9923-0 | URI: | http://hdl.handle.net/10261/248060 | DOI: | 10.1007/s11103-012-9923-0 | ISSN: | 0167-4412 |
Aparece en las colecciones: | (CRAG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
55
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
52
checked on 23-feb-2024
Page view(s)
58
checked on 18-abr-2024
Download(s)
10
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.